Figure 1. Age, sex, and overall health affect reference gene expression in skeletal muscle from C57BL/6 mice. A schematic diagram that illustrates the experimental approach used to compare reference gene stability in skeletal muscle (hamstrings) from young (4 mos) and old (25-26 mos) mice. Eight common reference genes were investigated (Gapdh, Gusb, Rplp0, B2m, Tubb5, Rpl7l1, Hprt, and Rer1). Health was assessed with a validated frailty index tool. Figure created with Biorender.com.