Research Paper Volume 15, Issue 10 pp 4122—4143

PCNT is a prognostic biomarker correlated with tumor immune microenvironment in hepatocellular carcinoma and promotes tumor progression by inhibiting cell cycle arrest


Figure 4. In vitro experiments validated PCNT expression and pro-oncogenic effects. (A) PCNT protein level in 4 paired HCC tissues and their adjacent normal liver tissues. (B) PCNT mRNA levels in LO2, Hep3B, HepG2, and Huh7 cell lines. (C, D) PCNT mRNA levels were down-regulated by shPCNT transfection in HepG2 (C) and Hep3B (D) cell lines. (E) Western blot assay validated the knockdown effects of shPCNT#2. (F, G) CCK-8 assays detected the cell viability of HepG2 (F) and Hep3B (G) cells. (H, I) The migration and invasion capacities were examined by transwell assays in HepG2 (H) and Hep3B (I) cell lines. *P < 0.05, **P < 0.01, ***P < 0.001.