Research Paper Volume 15, Issue 9 pp 3839—3856

Exploring the molecular mechanism of glycyrrhetinic acid in the treatment of gastric cancer based on network pharmacology and experimental validation


Figure 8. The effect of 18β-GRA on MAPK pathways in AGS cells. (A) qRT-PCR detection of KRAS, ERK1, and ERK2 expression levels. (B) Western blotting analysis revealed the presence of KRAS, ERK1/2, p-ERK1/2 protein expression levels. (C) The results quantify protein levels of KRAS, ERK1/2 and p-ERK1/2 used Image J software. (D, E) The effects of 18β-GRA on nuclear transfer of p-ERK1/2 in AGS cells and statistical chart. All the values are expressed as mean ± SD. Compared with the control group, **p < 0.01, ***p < 0.001.