Research Paper Volume 15, Issue 10 pp 4144—4158

Mechanisms of human umbilical cord mesenchymal stem cells-derived exosomal lncRNA GAS5 in alleviating EMT of HPMCs via Wnt/β-catenin signaling pathway


Figure 3. hUC-MSCs-CM alleviating EMT of HPMCs stimulated by HG through exosomes. (A) HG stimulation induced EMT of HPMCs, the hUC-MSC-CM mainly alleviated EMT through exosomes by Western Blot. (B) Statistical results of EMT changes after HG and hUC-MSCs/hUC-MSCs exosomes treatment. (C) The expression of EMT markers was detected by real-time PCR in HPMCs. (D) Treatment with hUC-MSCs exosomes effect cell morphology in HG-treated HPMCs. (E) The expression of lncRNA GAS5 was detected by real-time PCR. (F) The expression of miR-21 was detected by real-time PCR. (G, H) The expression of PTEN was detected by Western Blot. Each value represents the mean ± SEM (n = 3) (**P < 0.01 vs. Control, ##P < 0.01 vs. HG, #P < 0.05 vs. HG).