Research Paper Volume 15, Issue 10 pp 4182—4201
Sig1R activates extracellular matrix-induced bladder cancer cell proliferation and angiogenesis by combing β-integrin
Figure 2. BEM induces cell proliferation and angiogenesis in bladder cancer cells. (A) Cell viability of T24 and J82 cells incubated with BEM hydrogels (0, 5, 10, and 20 mg/mL) for 4 days was evaluated using the MTT assay. (B) SEM images of fibrous collagen structures in BEM hydrogels (5, 10, and 20 mg/mL). Scale bars = 100 nm. (C) Cell cycle distribution of bladder cancer cells treated with BEM hydrogels (0 and 20 mg/mL) for 48 h was evaluated by flow cytometry. The percentage of cells in each phase are shown. (E, F) HUVECs treated with T24 and J82 medium formed capillary-like structures in the, scale bar = 100 μm. *p < 0.05, **p < 0.01, ***p < 0.001.