Research Paper Volume 15, Issue 10 pp 4182—4201

Sig1R activates extracellular matrix-induced bladder cancer cell proliferation and angiogenesis by combing β-integrin


Figure 6. Sig1R promoted bladder cancer cell invasiveness in vivo and reduced overall survival. (A) Dissemination and metastasis of the bladder cancer cells in the zebrafish at 24 h post-injection and quantification of the number of disseminated foci. White boxes indicate disseminated and metastatic tumor lesions, scale bar = 5 μm. (B) xenograft in zebrafish for angiogenesis assay. White boxes indicate the regions of pictures shown in the right four pictures; the analysis of the subintestinal venous plexus was conducted. (C) The bladder cancer cells (T24 and J82) in the zebrafish at 24 h post-injection. (D) Kaplan-Meier analysis of overall survival in the zebrafish post-injection of each group. **p < 0.01, ***p < 0.001.