Research Paper Advance Articles

Sig1R activates extracellular matrix-induced bladder cancer cell proliferation and angiogenesis by combing β-integrin


Figure 8. β-Integrin mediates BEM-induced bladder cancer cell proliferation. (A) P5D2 treatment and control T24 and J82 cells were incubated with BEM hydrogels (0 and 20 mg/mL) for 2 weeks and allowed to form colonies. (B) The viability of treated P5D2 and control bladder cancer T24 and J82 cells incubated with BEM hydrogels (0 and 20 mg/mL) for 4 days was evaluated using the MTT assay. (C) P5D2 inhibited tumor-induced HUVEC angiogenesis according to tube formation assay in BEM, scale bar = 100 μm. (D) Quantification of VEGFA concentration in CM are shown. (E) Total protein extracts from NC, Sig1R-KD, or Sig1R-OE K562 cells plated on uncoated or BEM-coated dishes in the presence or absence of P5D2 were separated by SDS-PAGE and immunoblotted with Sig1R, E2F1, CCNE2, CDK2, PCNA, and VEGFA antibodies. **p < 0.01, ***p < 0.001.