Research Paper Volume 15, Issue 10 pp 4288—4303

DNMT1 regulates polarization of macrophage-induced intervertebral disc degeneration by modulating SIRT6 expression and promoting pyroptosis in vivo


Figure 4. DNMT1 in NPCs suppresses M1 polarization of co-cultured macrophages by regulating SIRT6. (AE) ELISA detection of the M1/M2 markers expression. (F, G) negative regulatory role of DNMT1 on SIRT6 expression. (H) GST pull-down and CO-IP experiments were used to detect the binding of DNMT1-SIRT6 and the CO-IP experiment of dnmt1-sirt6 interaction. (I) GST-pull down assay and immunoprecipitation assay were used to detect the interaction between SIRT6 and DNMT1. (J) the CD206 and CD86 for the macrophage phenotypes of all groups were detected by flow cytometry.