Research Paper Advance Articles

DNMT1 regulates polarization of macrophage-induced intervertebral disc degeneration by modulating SIRT6 expression and promoting pyroptosis in vivo


Figure 5. DNMT1/SIRT6 axis can affect the pyroptosis of nucleus pulposus cells by regulating macrophage polarization. (AE) The expression of M1/M2 macrophage specific markers detected by ELISA. (FK) Western blot and quantitative PCR to evaluate the transfection efficiency. (L) Heatmap of pyroptosis-related proteins expression which detected by western blot. (M) Transmission electron microscopy showed the autophagosomes in NPCs.