Figure 3.DNA replication, recombination, and repair genes are repressed during senescence. (A) Canonical pathway generated from the IPA analysis of the list of downregulated genes shows progressive changes of senescence-specific protein complexes associated with cell cycle control of chromosomal replication. Genes that are essential for pre-replication complex and initiation of DNA replication are decreased during senescence. Green denotes downregulated gene expression. (B) Expression levels of the genes RAD51, CDC6, and MCM6 were validated by RT-PCR in control, replicative senescence (RS) and TNF-α-induced senescence (TIS). GAPDH levels were used for normalization. Means ± SD are shown.
