Research Paper Volume 15, Issue 10 pp 4411—4428

Brevilin A shows an anti-tumor role in prostate cancer via the lncRNA H19/miR-194/E2F3 signaling pathway


Figure 5. The influence of lncRNA H19 and miR-194 on DU145 cell proliferation, apoptosis, invasion, and migration. H19 overexpression plasmids and si-H19 were transfected into DU145 cells, and miR-194 mimics or inhibitors were administered for 24 hours culture. (A, B) CCK8 assay detected cell proliferation. (C, D) Transwell monitored cell migration and invasion. Scale bar = 100 μm. (E, F) TUNEL staining examined apoptosis. Scale bar = 50 μm. (G, H) qRT-PCR examined the profiles of lncRNA H19 and miR-194. (I, J) Western blot was used for examining E2F3 protein level. **P < 0.01, ***P < 0.001 (vs. NC or siNC); &&P < 0.01, &&&P < 0.001 (vs. H19 or siH19), n = 3.