Research Paper Volume 15, Issue 10 pp 4411—4428

Brevilin A shows an anti-tumor role in prostate cancer via the lncRNA H19/miR-194/E2F3 signaling pathway


Figure 6. The impact of miR-194 and E2F3 on DU145 cell proliferation, apoptosis, invasion, and migration. E2F3 overexpression and si-E2F3 plasmids were transfected into DU145 cells for 24 hour culture after miR-194 mimics or inhibitors were administered. (A, B) CCK8 assay checked cell proliferation. (C, D) Transwell assay examined cell migration and invasion. Scale bar = 100 μm. (E, F) TUNEL staining detected cell apoptosis. Scale bar = 50 μm. (G, H) qRT-PCR examined the profiles of lncRNA H19 and miR-194. (I, J) Western blot was used for examining E2F3 protein level. ***P < 0.001 (vs. NC or NC-in); &P < 0.05, &&P < 0.01, &&&P < 0.001 (vs. miR-149 or miR-149 in), n = 3.