Research Paper Volume 15, Issue 10 pp 4035—4050

Changes in macrophage immunometabolism as a marker of skeletal muscle dysfunction across the lifespan


Figure 3. Clusters with clear trends of age-related decrease or increase reflect their origin signatures. (A, B) Feature plots and violin plots show signature scores of TRM or Monocyte-derived macrophage marker genes. Plots were generated using AddModuleScore function in R. Genes that were included as each signature score analysis are also indicated. Cell cluster of interest are indicated by *in the violin plot. (C) GO analysis of DEGs in young mice-specific (c0 and c2), Older mice-specific (c4 and c5), and Old mice-specific (c3 and c7) subclusters. GO terms included in c0 and c2 suggests young mice-specific macrophage populations are more phagocytic, anti-inflammatory macrophages. GO terms included in c4 and c5 suggests older mice-specific macrophage populations are involved in ECM synthesis and angiogenesis. GO terms included in c3 and c7 suggests old mice-specific macrophage populations are glycolytic, pro-inflammatory macrophages.