Research Paper Volume 15, Issue 12 pp 5381—5398

FBXO28 promotes proliferation, invasion, and metastasis of pancreatic cancer cells through regulation of SMARCC2 ubiquitination


Figure 1. FBXO28 is overexpressed in pancreatic cancer and is associated with poor prognosis. (A, B) In the GSE15471, GSE16515, GSE62165, and GEPIA2 datasets, pancreatic cancer (PC) tissue specimens had substantially higher levels of FBXO28 expression than paracancerous tissue specimens. (C) Based on data from The Cancer Genome Atlas database, Kaplan–Meier analysis reveals that FBXO28 expression is associated with overall survival (OS), disease-free survival (DFS), disease-specific survival (DSS), and progression-free survival (PFS) in patients with PC. (D, E) Typical IHC images show high expression of FBXO28 in pancreatic cancer tissues (magnification: ×50, ×200). (F) Kaplan–Meier survival curves for high and low expression of FBXO28 in patients with PC. (G, H) FBXO28 expression in PC and paracancerous tissues detected by qRT-PCR and western blot. (I, J) FBXO28 expression in five PC cells was examined using qRT-PCR and western blot analysis, with β-tubulin serving as a control variable. ***P < 0.001, ****P<0.0001.