Research Paper Volume 15, Issue 12 pp 5381—5398

FBXO28 promotes proliferation, invasion, and metastasis of pancreatic cancer cells through regulation of SMARCC2 ubiquitination


Figure 3. Overexpression of FBXO28 promotes invasion and migration of pancreatic cancer cells. (A) At 24 hours after scratching, the migratory capacity of PANC-1 and BxPC-3 cells was assessed using the wound healing assay. (B) Transwell assay evaluation of BxPC-3 and PANC-1 cell invasion and migration. (C) Western blot to verify the expression of FBXO28 and epithelial-mesenchymal transition (EMT)-related proteins. (D) The cells were injected into the spleen to establish a liver metastasis model in the mice according to grouping, and live imaging was performed. (E) Hematoxylin-and-eosin (HE) staining of liver metastases (magnification: ×50, ×200). **P < 0.01, ***P < 0.001, ****P < 0.0001.