Research Paper Volume 15, Issue 12 pp 5381—5398

FBXO28 promotes proliferation, invasion, and metastasis of pancreatic cancer cells through regulation of SMARCC2 ubiquitination


Figure 5. SMARCC2 upregulation reverses the effect of FBXO28 overexpression. (A) Western blot analysis demonstrating that SMARCC2 expression is lower in cells overexpressing FBXO28 and higher after FBXO28 knockdown. (BD) Cell Counting Kit-8 (CCK-8), EdU, and clone plate assays showing that SMARCC2 upregulation inhibits proliferation of FBXO28 overexpression-induced BxPC-3 cells. (E, F) Western blot and flow cytometry revealing that SMARCC2 upregulation inhibits FBXO28 overexpression-induced BxPC-3 cell cycle. (G, H) SMARCC2 upregulation inhibited FBXO28 overexpression-induced BxPC-3 cell invasion and migration. (I) Western blot to observe the changes in epithelial-mesenchymal transition (EMT)-related proteins in each group following SMARCC2 overexpression. *P < 0.05 **P < 0.01, ***P < 0.001.