Research Paper Volume 15, Issue 12 pp 5426—5444

Chaetocin exhibits anticancer effects in esophageal squamous cell carcinoma via activation of Hippo pathway


Figure 7. Chaetocin suppresses the growth of ESCC cell xenografts. (A) Representative photographs of a nude mouse model of xenograft tumors after control (vehicle), chaetocin (0.5 mg/kg) treatments were administered i.p. daily for 14 days. (B) Images of the tumor samples from each group. (C) The tumors of control mice and chaetocin-treated (0.5 mg/kg) mice on Day 15 are shown. (D) Tumor volumes were measured and calculated every other day. (E) Tumor mass was calculated. Error bars represent SD. ***P < 0.001 compared with the control using one-way analysis of variance. (F) Body weight during administration of chaetocin. (G) Relative organ weights after two weeks of treatment with chaetocin. (H) Western blot was performed to detect the protein expression of PARP, Cleaved PARP, YAP, p-YAP (S127), and caspase-3 in xenograft tumor. GAPDH was used as the loading control. (I) Representative images of H&E staining for pathological examination of different tissues. (J) Immunohistochemical staining for Ki67, cleaved caspase-3, YAP and p-YAP (S127) in tumor sections. Data are expressed as mean ± SD (n = 8). *P < 0.05, **P < 0.01 compared with the control group.