Research Paper Volume 15, Issue 12 pp 5550—5568

Mettl3-mediated m6A modification plays a role in lipid metabolism disorders and progressive liver damage in mice by regulating lipid metabolism-related gene expression


Figure 3. Hepatocyte-specific deletion of Mettl3 induces lipid accumulation in mouse liver cells. (A) qRT-PCR assay of the relative Mettl3 expression in control and METTL3 HKO mouse livers at 4 and 12 weeks. (B) Representative Western blot analysis and quantitative results in control and METTL3 HKO mouse livers. (CF) Body weight (C), liver weight (D), and liver-to-body weight ratio (E) of METTL3 HKO and control mice at 4 and 12 weeks. (F) Representative images of the livers of METTL3 HKO (n = 5 for 4 weeks; n = 10 for 12 weeks) and control mice (n = 6 for 4 weeks; n = 8 for 12 weeks) stained with hematoxylin and eosin and Oil red O. Scale bars, 25 μm. (GK) Serum levels of ALT (G), AST (H), TG (I), TC (J), and Oil red O positive area (K). Data are presented as mean ± SD. Abbreviations: TG: triglyceride; TC: total cholesterol; ns: not significant; *P < 0.05; **P < 0.01; ***P < 0.001.