Research Paper Volume 15, Issue 12 pp 5662—5672
Exploration of the effect of PUM1/Cripto-1 pathway on ferroptosis by regulating macrophage polarization in allogeneic blood transfused mice
Figure 3. (A) The results of detecting total iron content, Fe2+ content, ROS, MDA and GSH content by corresponding test kits. The specific values were: 7.77 ± 0.40 nmol (Total iron), 3.18 ± 0.11 nmol (Fe2+), 2101.55 ± 194.88 RLU/mgprot (ROS), 12.24 ± 1.76 nmol/mL (MDA), 542.25 ± 40.64 μmol/L (GSH). (B) The results of Western blot analysis of protein GPX4 and Nrf2 content. The symbol * means p < 0.05 compared to Control group. The symbol # means p < 0.05 compared to ABT group. The symbol and means p < 0.05 compared to ABT+si-PUM1 group.