Research Paper Volume 15, Issue 12 pp 5713—5733

MAGOH is correlated with poor prognosis and is essential for cell proliferation in lower-grade glioma

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Figure 7. In vitro confirmation of the expression of MAGOH in LGG. (A) Western blot analysis of MAGOH expression in LGG tissues and corresponding para-carcinoma tissues. (B) Western blot and (C) qRT-PCR analysis of MAGOH expression in NHA and LGG cell lines. (D) Viability of SW1088 cells transfected with si-MAGOH or si-NC, as determined by CCK-8 assays. (E, F) Effect of MAGOH knockdown on colony formation by SW1088 cells. (G, H) Cell cycle distributions of SW1088 cells transfected with lentiviruses encoding si-MAGOH or si-NC. (I, J) Representative images (I) and histogram analysis (J) of EdU assays after silencing MAGOH in SW1088 cells. *P < 0.05, **P < 0.01, ***P < 0.001.