Research Paper Volume 15, Issue 12 pp 5734—5750

lncSNHG3 drives breast cancer progression by epigenetically increasing CSNK2A1 expression level


Figure 2. STAT3 activation induce high SNHG3 expression in BC. (A, B) SNHG3 expression was detected by qRT-PCR in MDA-MB-231 and BT549 cells transfected with the STAT3 siRNAs or STAT3 overexpression vector. (C) ChIP assays were performed to detect STAT3 occupancy in the SNHG3 promoter region. (D) Luciferase reporter assays were used to determine the STAT3 binding sites on the SNHG3 promoter region. (E) Expression of STAT3 in TCGA BC cohorts. (F) qRT-PCR analysis of STAT3 expression in 40 pairs of BC and corresponding normal tissues. (G) The correlation between the STAT3 and SNHG3 expression levels were analyzed in 40 paired BC samples.