Research Paper Volume 15, Issue 12 pp 5734—5750

lncSNHG3 drives breast cancer progression by epigenetically increasing CSNK2A1 expression level


Figure 5. SNHG3 regulates CSNK2A1 expression in BC. (A) Volcano plot showing aberrantly expressed genes after knockdown of SNHG3 in MDA-MB-231. (B) GSEA results were plotted to visualize the pathways related to SNHG3 regulated genes. (C) CSNK2A1 expression was detected by qRT-PCR in MDA-MB-231 and BT549 cells transfected with the SNHG3 siRNAs. (D) Expression of CSNK2A1 in TCGA BC cohorts. (E) qRT-PCR analysis of CSNK2A1 expression in 40 pairs of BC and corresponding normal tissues. (F) The correlation between the CSNK2A1 and SNHG3 expression levels were analyzed in 40 paired BC samples. (G) Representative images for HE-staining, CSNK2A1 immunostaining of tumor samples from the different groups. (H) Relative SNHG3 expression levels in nuclear and cytosolic fractions of MDAMB-231 and BT549 cells. Nuclear controls: U6, cytosolic controls: GAPDH. (I) Representative FISH images showed the expression of SNHG3 in MDA-MB-231 and BT549 cells (red). Nuclei were stained by DAPI (blue).