Research Paper Volume 15, Issue 13 pp 6331—6345

Figure 2. MIR34A directly targets and represses the expression of HK1. (A) A Bioinformatics-based target analysis showed that HK1 is a potential target of MIR34A. (B) The 293T cells were transfected with MIR34A and HK1 3’UTR WT co-transfected into 293T cells. Luciferase reporter assay showed that the luciferase activity of HK1 3′UTR-WT significantly decreased with MIR34A mimic transfection, compared to that of the NC mimic or HK1 3′UTR-mutant group. (C) RT-qPCR analysis was performed to detect the expression of HK1 after MIR34A mimics were transfected into SRA01/04 cells at 48 h, 96 h, and 120 h. (D) SRA01/04 cells were performed as C, and the expression of HK1 was detected by western blot. ^*P < 0.05; ^***P < 0.001.