Research Paper Volume 15, Issue 14 pp 7084—7097

Figure 1. A disrupted immune landscape in the lung of mice recovered from severe pneumonia. (A) Schematic of the experimental timeline. Untreated (UNTX, mice not treated with LPS) and LPS-treated mice were then monitored for blood oxygen, and samples were collected for molecular pathological assessment. (B) Body weight of untreated and LPS-treated mice. (C) Blood oxygen saturation data of untreated and LPS-treated mice. (D) Inflammatory factors, including IL-1β, IL-6, and TNF-α, in lung tissue homogenate. (E) Representative Masson staining of lung sections after various treatments as indicated. Scale bars, 200 μm. (F) Representative plots of CD3⁺ cells as a percentage of the total cell population in lung tissues and (G) corresponding quantification results. (H) Representative flow cytometric analysis of T cells and quantitation of the percentage of CD8⁺ and CD4⁺ cells among CD3⁺ T cells in lung tissues. (I) Representative flow cytometry chart of naïve and memory CD8⁺ T cells in lung tissues and corresponding quantification results. (J) Representative flow cytometry chart of naïve and memory CD4⁺ T cells in lung tissues and corresponding quantification results. Data are shown as the mean ± SD (n=4). Statistical significance was calculated by Student’s t test (two-tailed) and one-way ANOVA using the Tukey posttest. *P < 0.05; **P < 0.01; ***P < 0.001; n.s. nonsignificant.