Figure 4. Recovered mice showed a low antigen response systemically. (A) Schematic of the experimental timeline. Untreated and recovered mice were immunized with KLH antigen, and the paw swelling was observed and serum ELISA was performed. (B) KLH delayed-type hypersensitivity data after sensitization of untreated and recovered mice (n=5). Footpad swelling at 48 h after challenge. (C) KLH antibodies measured by ELISA one month after challenge (n=4). Data are shown as the mean ± SD. Statistical significance was calculated by Student’s t test (two-tailed) and one-way ANOVA using the Tukey posttest. *P < 0.05; **P< 0.01; ***P < 0.001.