Research Paper Volume 15, Issue 15 pp 7637—7654
TMT-based quantitative proteomic analysis revealed that FBLN2 and NPR3 are involved in the early osteogenic differentiation of mesenchymal stem cells (MSCs)
Figure 4. Validation of proteomic sequencing. (A) In all, 4 adipogenic differentiation-related proteins were validated by western blot (WB); (B) 4 osteogenic differentiation-related proteins were validated by western blot (the protein marked in the red box will undergo further examination); (C) Lentivirus-mediated shRNA delivery for the effective knockdown of FBLN2 or NPR3; (D) ARS displayed a decrease in the osteogenic ability of MSCs induced by FBLN2 or NPR3 deficiency; (E) Quantitative analysis of alizarin red staining (ARS) following knockdown of FBLN2 or NPR3.