Research Paper Volume 15, Issue 21 pp 11782—11810

Hub gene identification and molecular subtype construction for Helicobacter pylori in gastric cancer via machine learning methods and NMF algorithm


Figure 2. Construction of a NMF subtype based on the differentially expressed HP-related genes in the TCGA-STAD cohort. (A) NMF consensus clustering for k = 2. (B) Kaplan–Meier analysis of overall survival (OS) for Cluster C1 and C2. (C) Principal component analysis (PCA). (D, E) Differential analyses of immune and stromal score between Cluster C1 and C2. (F) Violin plot showing the immune cell infiltration landscape across different clusters. (G, H) Box plot of estimated IC50 values for Imatinib and Sunitinib in Cluster C1 and C2. (I) Box plot visualizing the significant expression differences of immune checkpoints across distinct clusters, including BTLA and PD-L2. *:P<0.05 ** :P<0.01 ***:P<0.001.