Research Paper Volume 15, Issue 21 pp 12330—12368

A robust six-gene prognostic signature based on two prognostic subtypes constructed by chromatin regulators is correlated with immunological features and therapeutic response in lung adenocarcinoma


Figure 4. Molecular prognostic subtypes construction based on 27 key prognostic differentially expressed chromatin regulators (DECRs) and the associations with overall survival (OS) of patients in lung adenocarcinoma (LUAD). (AC) TCGA-LUAD. (DF) GSE31210. (GI) GSE50081. (JL) GSE68465. (MO) GSE72094. (A, D, G, J, M) Cumulative distribution function (CDF) curve and CDF delta area curve. Clustering stability increasing curves were plotted from k = 2 to 9 for five gene expression datasets including TCGA-LUAD, GSE31210, GSE50081, GSE68465 and GSE72094. (B, E, H, K, N) Consensus clustering heat map. The k = 2 was selected to cluster samples for each of five gene expression datasets, and LUAD patients were divided into two clusters. (C, F, I, L, O) Survival curve. Two clusters were significantly associated with the OS of LUAD patients in five independent datasets (p = 0.016, < 0.0001, = 0.008, = 0.00038, = 0.00055, respectively).