Research Paper Volume 15, Issue 21 pp 12413—12450

Construction and validation of a novel angiogenesis pattern to predict prognosis and immunotherapy efficacy in colorectal cancer


Figure 11. Influences of KLK10 expression on CRC cell proliferation, migration, and invasion. (A) The efficacy of the KLK10 transcript was detected after KLK10 knockdown in two CRC cell lines. (B) Cell viability was assessed by CCK8 array when KLK10 expression was reduced in cells. (C) Wound-healing assay of CRC cells with KLK10 knockdown monitored for 48-h with 24-h intervals. (D) Correlations between KLK10 expression and EMT markers (E-cadherin, N-cadherin, and Vimentin) using Spearman analysis. (E, F) The protein levels of E-cadherin, N-cadherin, and Vimentin were measured by western blotting after KLK10 knockdown in two CRC cell lines. Data are presented as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001.