Research Paper Volume 15, Issue 21 pp 12413—12450

Construction and validation of a novel angiogenesis pattern to predict prognosis and immunotherapy efficacy in colorectal cancer


Figure 12. CRC cells co-cultured with F.n were found to have significantly upregulated KLK10 expression. (A) The Venn diagram demonstrating that KLK10 was shared by F.n infection-related DEGs and prognostic ARGs. (B) Microarray analysis of KLK10 expression between HCT116 cells and F.n infected HCT116 cells (left panel) and expression of KLK10 in HT29 cells incubated with or without F.n (right panel). (C, D) The KLK10 mRNA expression in CRC cell lines infected with or without F.n for 24 h and 48 h, respectively, was determined by qPCR. (E) Representative western blot for KLK10 protein and β-actin protein extracted from CRC cells infected with F.n for 0, 12, 24, and 48 h. *P < 0.05; **P < 0.01; ***P < 0.001.