Research Paper Volume 15, Issue 22 pp 12873—12889

Silencing of Dicer enhances dacarbazine resistance in melanoma cells by inhibiting ADSL expression


Figure 3. Association of ADSL expression with DTIC response in melanoma cells. (A) Western blotting results for A375 and A2508 melanoma cells treated with DTIC at the indicated concentrations. (B) Western blotting results for metabolic pathway–associated enzymes in wild-type and Dicer-silenced A375 cells. (C) Western blotting results indicating ADSL and Dicer levels in wild-type, shctrl, and ADSL-silenced (shADSL #1 and #2) A375 cells. (D) MTT assay results indicating the viability of ADSL-silenced A375 cells after incubation with DTIC for 48 h. (E) Expression levels of Oct-4, Nanog, SOX2, and KLF4 mRNA (measured through qRT-PCR and Western blotting). **P < 0.01 and ***P < 0.001 (Student’s t test).