Research Paper Volume 15, Issue 22 pp 13077—13099
Identification of tryptophan metabolism-related genes in immunity and immunotherapy in Alzheimer’s disease
Figure 9. Validation of 5 gene markers in APP/PS1 mice. (A–E) qPCR to determine the mRNA expression of PCBB (A), FARSB (B), EZR (C), NFASC (D), and TEAD1 (E). (F) Western blotting to determine the protein expression levels of PCCB and NFASC. (G, H) Immunofluorescence to assess the expression of PCCB (G) and NFASC (H) in the hippocampus and cerebral cortex.