Research Paper Volume 15, Issue 23 pp 13680—13692

Figure 4. Involvement of TXNIP in APN-inhibited human NP cell pyroptosis. (A) Human NP cells were treated with PBS, empty vector or lentiviral vector expressing TXNIP for 24 h, followed by western blot analysis of TXNIP protein. (B–E) Human NP cells were transfected with APN and/or TXNIP by lentiviral vector in the presence of LPS. (B) Cell lysates were immunoblotted with indicated antibodies. (C) The levels of IL-1β and IL-18 were detected using ELISA in the cell culture supernatants. (D) The LDH assay kit was employed to measure LDH activity in the cell culture supernatants. (E) Representative images of fluorescent staining with Hoechst 33342 (blue) and PI (red). PI positive cells were quantified. Data shown are mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.