Research Paper Volume 15, Issue 23 pp 13738—13752
Involvement of RFC3 in tamoxifen resistance in ER-positive breast cancer through the cell cycle
Figure 7. Effect of RFC3 overexpression on MCF-7 cells. (A) qPCR assay showed that RFC3 mRNA expression was significantly increased in MCF-7 cells overexpressing RFC3 compared to the control group. (B) WB assay showed that RFC3 protein expression was significantly increased in RFC3-overexpressing MCF-7 cells compared with the control group. (C) CCK-8 assay showed that the proliferation rate of RFC3-overexpressing MCF-7 cells was significantly increased after TAM treatment compared to the control group. (D, E) Flow cytometry apoptosis assay showed that apoptosis was significantly reduced in RFC3-overexpressing MCF-7 cells after TAM treatment compared to the control group.