Research Paper Volume 15, Issue 23 pp 13920—13943

Figure 8. In vivo, hsa-miR-300 regulates tumor growth through CDKN1A. (A) After the treated cells were inoculated, the weight of the mice was measured weekly. (B) Four weeks later, the mice were sacrificed to separate the tumor tissue, the diameter was measured and photos were taken. (C, D) After inoculation, tumor diameter and weight were measured weekly during tumor growth using vernier calipers and scale. (E, F) The expression of CDKN1A, CDKN2A, TP53 and GADD45B in tissue homogenate was detected. A total of 9 nude mice were included in the experiment and divided into control groups, miR-300 (-)/CDKN1A (-) and miR-300 (+)/CDKN1A (+) groups, where (-) represents gene interference and (+) represents gene expression. Each mouse was inoculated with 5 x 10⁶-1 x 10⁷ cells for axillary injection. * p-value < 0.05, ** p-value < 0.01, *** p-value < 0.001.