Research Paper Volume 16, Issue 3 pp 2438—2456
Interaction of Sp1 and Setd8 promotes vascular smooth muscle cells apoptosis by activating Mark4 in vascular calcification
Figure 5. Sp1 interacted with Setd8 to regulate Mark4 expression in VSMCs. (A) Several proteins that interacted with Sp1 were shown (https://string-db.org). (B) Immunohistochemistry of Sp1 and Setd8 in CKD patients with or without arterial calcification. Scale bars: 50 μm. (C) RT–qPCR analysis of mRNA expression of Sp1 and Setd8 in serum from CKD patients with (n = 11) or without VC (n = 10). ***Means p < 0.001 vs. patients without VC group. (D) Correlation analysis between the expression of Setd8 mRNA and Sp1 mRNA in CKD patients (n = 21, the Pearson’s correlation coefficient r value and the p-value are shown). (E) Representative IF images showing the colocalization of Sp1 and Setd8. Scale bars: 20 μm. (F, G) The association between Sp1 and Setd8 in VSMCs was confirmed by Co-IP.