Research Paper Volume 16, Issue 3 pp 2702—2714

MicroRNA-146a gene transfer ameliorates senescence and senescence-associated secretory phenotypes in tendinopathic tenocytes


Figure 1. miR-146a expression in tendon tissues from human long head of biceps (LHB) with tendinopathic changes. (A) Representative figures of in situ hybridization of miR-146a in LHB tendons with different severity grades. Arrow heads indicate miR-146a- or U6-positive cells. Scale bars represent 50 μm in × 400 magnifications. The higher-magnification views of the red-boxed areas are shown beneath them. (B) miR-146a-positive cells were counted and normalized with U6-counterstained total cells. Spearman correlation rank test was used. (C) miR-146a expression levels in tendon tissues of normal hamstring (Ham) tendon during anterior cruciate ligament reconstruction and LHB, as determined by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Values are represented as the mean ± standard error of the mean (SEM). n=10 each, ***p<0.001.