Research Paper Volume 16, Issue 3 pp 2715—2735

Integrated single-cell and bulk characterization of branched chain amino acid metabolism-related key gene BCAT1 and association with prognosis and immunogenicity of clear cell renal cell carcinoma


Figure 1. Prognostic and biological characteristics of the branched chain amino acids (BCAA) metabolism-related clusters. (A) Results of univariate cox analysis of BCAA metabolism-related genes. (B) Consensus map for non-negative matrix factorization (NMF) clustering. (C) The Kaplan-Meier (KM) survival curve of BCAA metabolism-related clusters. (D) Heatmap showing the correlations with clinicopathological characteristics based on results of the cluster analysis. (E) Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment of differentially expressed genes (DEGs) between clusters A and B. The enriched items were analyzed by using gene counts and adjusted p-values. (F) Gene ontology (GO) functional annotation analysis of DEGs between clusters A and B, including enriched biological processes (BP), cellular components (CC), and molecular functions (MF). (G) Results of Gene Set Variation Analysis (GSVA) enrichment analysis between clusters.