Figure 2. CWH22 and CLM22 organoids maintain histological and proliferative characteristics of the corresponding tumors. (A) H&E staining of the tumor organoids showing the resemblance of the organoids to the lumens and tubular structures of the primary tumor. IHC staining demonstrated consistent positive expression of CK20 and CDX2 in the organoids and corresponding tumors (scale bars, 100 μm). (B) IHC staining of CWH22 and CLM22 organoids demonstrating the consistent expression of p53, Ki67, MLH1, PMS2, MSH2, MSH6, and Villin, with the expression in CRC and CLM tissues (