Research Paper Volume 16, Issue 5 pp 4396—4422
A pair of primary colorectal cancer-derived and corresponding synchronous liver metastasis-derived organoid cell lines
Figure 7. Tumorigenicity of adherent CWH22-2D and CLM22-2D cells. (A) Bright-field images of CWH22-2D/CLM22-2D cells (left panel); and immunofluorescent staining images of CK20 and CDX2 (middle and right panels) from CWH22-2D/CLM22-2D cells. Scale bars, 50 μm. (B) Subcutaneous tumor growth curves were plotted according to the monitored tumor size every seven days for 35 days. The tumors were derived from CWH22-2D/CLM22-2D cells and CWH22/CLM22 organoids. (C) Bioluminescence imaging of mice with liver metastases 42 days after splenic injection of CWH22-2D and CLM22-2D cells, and livers with metastatic lesions harvested from mice receiving splenic injections of CWH22-2D and CLM22-2D cells. (D) Representative image of the entire liver lobe with metastases, and focal magnification. Scale bars, 100 μm.