Withdrawal Notice

This paper was originally published in Aging Advance Online Publications on March 14, 2024.

In compliance with Aging's withdrawal policy, the paper was withdrawn in its entirety. It will not appear in Aging internal or any external indexes or archives.

WITHDRAWN: Metformin mitigates cisplatin-induced ovarian damage through inhibiting the pyroptosis of granulosa cells via ROS/TXNIP/NLRP3 signaling pathway


Figure 5. Reversal of cisplatin-induced granular cell pyroptosis by metformin. (A, B) Percentage of granulosa cell proliferation and pyroptosis in each group. (C) Transmission electron microscopy images showed subcellular morphology of mouse ovaries treated with different methods (cisplatin or metformin). Notes: Black arrow: mitochondria; Blue arrow: endoplasmic reticulum; Red arrow: nucleus of the granular cell. (D) Real-time PCR quantitative analysis of the expression levels of oxidative stress related genes in each group. (E, F) Western blot analysis of Caspase-1, GSDMD, IL-1β, and TNF-α protein expressions in ovaries treated with different medication regimens. *p < 0.05, **p < 0.01. (G) Comparison of the number of granulosa cell pyroptosis after different intervention methods. (H) Changes in gene expression of granulosa cell inflammation after different intervention methods.