Research Paper Volume 17, Issue 7 pp 1784—1809

Figure 5. Age-associated proteins secreted by the young BM model rejuvenate skin cells. Old (>60 years) human primary dermal fibroblasts and old (>60 years) human primary epidermal keratinocytes were statically cultured and treated with 100 ng/ml of the appropriate downregulated age-associated protein for 72 hours. (A) Immunofluorescence staining of Ki67 of fibroblasts (top) and keratinocytes (bottom). Bar graphs show the relative proportion of Ki67+ cells normalized to the corresponding untreated control, n = 5–20. (B) Heatmap indicating relative gene expression of statically cultured fibroblasts treated with proteins normalized to the untreated control, n = 4. (C) Bar graph illustrating the relative production of hyaluronic acid normalized to the untreated control, measured in the supernatant of fibroblasts, n = 3–7. (D) Bar graph showing the relative ability of fibroblasts to differentiate into adipocyte-like cells of fibroblasts treated with proteins normalized to the control cultured without proteins, n = 7–14. (E) Bar graph showing the relative production of procollagen 1 normalized to the untreated control, measured in the supernatant of fibroblasts, n = 3–7. (F). Bar graph illustrating the relative mitochondrial membrane potential of treated fibroblasts normalized to the corresponding untreated control, n = 6–10. Data are shown as mean values +/− SEM. Paired t-test, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001.