Research Paper Advance Articles
Methylglyoxal-induced glycation stress promotes aortic stiffening: putative mechanistic roles of oxidative stress and cellular senescence
Figure 3. MGO-induced glycation stress drives aortic stiffness, in part, by increasing reactive oxygen species (ROS) levels. (A) Superoxide levels assessed via Electron Paramagnetic Resonance spectroscopy in young intervention-naïve male mouse aortic rings following exposure to standard media (control), MGO, and MGO+Gly-Low (n=3/group). (B) Elastic modulus in aortic rings incubated with/without superoxide scavenger TEMPOL (n=5/group). (C) Mechanistic schematic depicting how MGO-induced glycation stress causes aortic stiffness via increasing ROS levels. Panels (A, B) were analyzed using paired t-tests, as different media conditions were tested on aortas obtained from the same mouse. All values are in mean ± SEM, *p<0.05 vs. control; ♦p<0.05 vs. MGO alone.