Research Paper Volume 15, Issue 20 pp 11554—11570
Comprehensive analysis of transcriptome data and experimental identification show that solute carrier 35 member A2 (SLC35A2) is a prognostic marker of colorectal cancer
- 1 Department of Pathology, School of Basic Medical Science, Anhui Medical University, Hefei, Anhui, People’s Republic of China
- 2 First Hospital of Jiaxing, Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang, People’s Republic of China
- 3 Department of Pathology, Fuyang Hospital Affiliated to Anhui Medical University, Fuyang, Anhui, People’s Republic of China
Received: February 3, 2023 Accepted: October 2, 2023 Published: October 26, 2023
https://doi.org/10.18632/aging.205145How to Cite
Copyright: © 2023 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Background: Colorectal cancer (CRC) is a solid tumor with high morbidity and mortality rates. Accumulating evidence shows that the soluble carrier family 35 member A2 (SLC35A2), a nucleotide sugar transporter, plays a key role in the pathogenesis of various tumors. However, its expression and function in CRC has not been fully elucidated.
Methods: The prognosis-related gene SLC35A2 was obtained using differential analysis, prognosis correlation analysis, and LASSO regression screening. Its expression levels in CRC tissues were analyzed, and so was the relationship of this expression with clinical characteristics of patients. Subsequently, the expression levels were correlated with clinicopathological parameters using immunohistochemical analysis. Analysis based on GO/KEGG databases was used to reveal the potential mechanisms of SLC35A2. Next, we explored the relationship between SLC35A2 and immune cells in CRC tissues. A nomogram was created to help understand the prognosis of CRC patients. Finally, western blotting and qRT-PCR reaction were used to verify the expression of SLC35A2 in CRC cell lines.
Results: SLC35A2 expression was upregulated and related to tumor pathological stage and lymph node metastasis, indicating that SLC35A2 is an independent prognostic factor and a potential diagnostic marker for CRC. We verified by IHC, WB and PCR that the expression of SLC35A2 was up-regulated in colorectal cancer tissues and cell lines, and its high expression was related to the tumor pathological stage of CRC clinical samples.
Conclusions: Our study found that SLC35A2 can be used as a biomarker for the diagnosis and prognosis of CRC, providing motivation for further study.
Abbreviations
CRC: colorectal cancer; HCC: hepatocellular carcinoma; SLC35A2: soluble carrier family 35 member A2; LASSO: least absolute shrinkage and selection operator; GO: Gene Ontology; KEGG: Kyoto Encyclopedia of Genes and Genomes; IHC: immunohistochemistry; CEA: Carcinoembryonic antigen; NST: nucleotide sugar transporters; UDP: Uridine diphosphate; TCGA: The Cancer Genome Atlas; GEO: Gene Expression Omnibus; BP: biological processes; CC: cellular components; MF: molecular functions; BLCA: Bladder Urothelial Carcinoma; BRCA: Breast invasive carcinoma; CHOL: Cholangio carcinoma; COAD: Colon adenocarcinoma; ESCA: Esophageal carcinoma; HNSC: Head and Neck squamous cell carcinoma; KIRC: Kidney renal clear cell carcinoma; LIHC: Liver hepatocellular carcinoma; LUAD: Lung adenocarcinoma; LUSC: Lung squamous cell carcinoma; PRAD: Prostate adenocarcinoma; READ: Rectum adenocarcinoma; STAD: Stomach adenocarcinoma; UCEC: Uterine Corpus Endometrial Carcinoma; THCA: Thyroid carcinoma; CDG: congenital glycosylation disease; IFCD: congenital glycosylation disease; RT-qPCR: Quantitative real-time polymerase chain reaction; TIMER2: Tumor Immune Estimation Resource.