Research Paper Volume 11, Issue 17 pp 7274—7293

Figure 3. Effect of cardiac dSir2 knockdown on young hearts. (A) Cardiac dSir2 expression level. (B) Cardiac Foxo expression. (C) Cardiac SOD activity level. (D) Cardiac MDA level. (E) Cardiac TAG level. (F) Cardiac bmm expression. (G) Heart period. (H) Heart systolic period. (I) Heart diastolic period. (J) Heart diastolic dysfunction index. The diastolic dysfunction index is diastolic interval standard deviation/diastolic interval median). (K) Fractional shortening. (L) Diastolic diameter. (M) Systolic diameter. (N) Microscopic image of cardiac function from M-mode trace. 1: hand-Gal4>w¹¹¹¹⁸ flies; 2: hand-Gal4>UAS-dSir2^RNAi flies; 3: 7-week-old hand-Gal4>w¹¹¹¹⁸ flies; 4: 7-week-old hand-Gal4>UAS-dSir2-RNAi flies. It can be observed from 1 and 2 that the cardiac dSir2 knockdown could reduce heart period and fractional shortening, and increase diastolic dysfunction. Independent-sample t tests were used to assess differences between the hand-Gal4>w¹¹¹¹⁸ and hand-Gal4>UAS-dSir2^RNAi flies to explore the effects of cardiac dSir2 knockdown on the heart. Data are represented as means ± SEM. *P<0.05; **P <0.01. Sample size was the same as in our previous experiments.