Research Paper Volume 12, Issue 13 pp 12750—12770

Navitoclax (ABT263) reduces inflammation and promotes chondrogenic phenotype by clearing senescent osteoarthritic chondrocytes in osteoarthritis


Figure 7. Improvement of inflammation and promotion of proteoglycan anabolism. (A) immunohistochemical analysis of senescence and inflammation-related markers and safranine O staining after 21-day pellet culture. Scale bar: 200 μm. (B) mRNA expression analysis by qPCR for CDKN1A, CDKN2A, MMP13, ADAMTS5, IL1β, IL6, COLII, ACAN and SOX9. (C) protein level analysis using Immunoblotting for C-Caspase3, p21, MMP13, ADAMTS5, IL1β, COLII, ACAN and SOX9. Data are shown as mean ± standard deviation. N = 3 per group.