Research Paper Volume 12, Issue 13 pp 12750—12770

Navitoclax (ABT263) reduces inflammation and promotes chondrogenic phenotype by clearing senescent osteoarthritic chondrocytes in osteoarthritis


Figure 8. Senescent cells clearance by treatment with ABT263 after rat DMM surgery attenuates the injury of post-traumatic OA. (A) Schematic of the time course for the experiments in b–d. Male SD rat that underwent DMM surgery were injected intra-articularly every other day with vehicle (Veh) or ABT263 for two weeks and evaluated as indicated. (B) Representative images of Hematoxylin and eosin staining and safranin O and methyl green staining of the medial femoral condyle and medial tibial plateau in rat right knee. Scale bar: top: 500 μm; bottom: 100μm. (C) OARSI scores of the knee joints of rat with DMM or sham surgery. N = 4 per group. (D) Sagittal micro-CT and three-dimensional reconstruction images of the hind knee joint from different groups. ABT263 of 1.0 mM was corresponded to the results presented in B, C and D; Data are shown as mean ± standard deviation. Scale bar: 2 mm.