Research Paper Volume 12, Issue 18 pp 17921—17929

circKIF4A sponges miR-127 to promote ovarian cancer progression


Figure 3. circKIF4A acts as a sponge for miR-127. (A) The expression levels of nuclear control (18S), cytoplasmic control (GAPDH) and circKIF4A were detected. (B) The predicted binding sites of miR-127 within the circKIF4A sequence. (C) Luciferase assay of cells co-transfected with miR-127 mimics and wild type or mutant luciferase reporter. (D) MS2-based RIP assay transfected with MS2bs-circKIF4A, MS2bs-circKIF4Amt or control. **P < 0.01.