Research Paper Volume 12, Issue 13 pp 13400—13421

Penehyclidine hydrochloride inhibits renal ischemia/reperfusion-induced acute lung injury by activating the Nrf2 pathway


Figure 10. Nrf2 dependence of the effects of PHC on NF-κB/NLRP3 signaling in the lungs following rI/R in rats. WT and Nrf2−/− rats were used to model rI/R-induced acute lung injury, and lung sections were obtained 24 h after rI/R treatment. Lung homogenization was used to obtain the total protein, nuclear and cytoplasmic extracts. Western blotting was used to measure the protein levels of ASC, NLRP3, pro- and mature IL-1β, caspase-1 p10, IκBα and p-IκBα, along with the nuclear concentration of p65. GAPDH was used as an internal control for cytoplasmic and total proteins, while histone H3.1 was used as an internal control for nuclear proteins. Data are presented as the mean ± S.D. (n = 8). *P < 0.05, **P < 0.01 vs. the sham group. #P < 0.05, ##P < 0.01 vs. the rI/R group.