Research Paper Volume 12, Issue 18 pp 17976—17989

Prothrombotic effects of high uric acid in mice via activation of MEF2C-dependent NF-κB pathway by upregulating let-7c


Figure 2. HUA increases let-7c expression to stimulate hemagglutination and thrombosis in mice. Normal mice were used as controls, and HUA mice were treated or not treated with NC-inhibitor or let-7c-inhibitor. (A) SUA in serum of mice detected using Urea Assay Kit. (B) Let-7c expression in serum of mice detected by RT-qPCR normalized to U6. (C) Fibrinogen level in serum of mice determined by ELISA. (D) D-dimer level in serum of mice determined by ELISA. (E) western blot analysis of CD36 and CD41 in platelets of mice normalized to β-actin. * p < 0.05 vs. normal mice. # p < 0.05 vs. HUA mice treated with NC-inhibitor. The measurement data were shown as mean ± standard deviation and compared by one-way analysis of variance, followed by Tukey's post hoc test. n = 10.